منابع مشابه
Mismatch repair in heteroduplex DNA.
DNA with base pair mismatches was prepared by annealing mixtures of genetically marked DNA from bacteriophage lambda. This heteroduplex DNA was used to transfect bacteria under conditions minimizing recombination. Genetic analysis of the progeny phages indicates that: (i) Mismatch repair occurs, usually giving rise to a DNA molecule with one chain with the genotype arising from repair and one p...
متن کاملMismatch - stimulated killing ( bacteriophage A / heteroduplex DNA / DNA methylation / mlsmatch repair / double - strand break )
DNA duplexes with or without mismatches and with or without adenine-methylated GATC sequences were prepared from separated strands of bacteriophage A DNA and used to transfect Escherichia cofi. Unmethylated heteroduplexes containing one or more repairable mismatches transfect cells with a functioning mismatch repair system less efficiently than they transfect cells deficient in mismatch repair....
متن کاملIdentification of mismatch repair protein complexes in HeLa nuclear extracts and their interaction with heteroduplex DNA.
Deficiencies in DNA mismatch repair (MMR) have been found in hereditary colon cancers (hereditary non-polyposis colon cancer, HNPCC) as well as in sporadic cancers, illustrating the importance of MMR in maintaining genomic integrity. We have examined the interactions of specific mismatch repair proteins in human nuclear extracts. Western blot and co-immunoprecipitation studies indicate two comp...
متن کاملHeteroduplex DNA and ATP induced conformational changes of a MutS mismatch repair protein from Thermus aquaticus.
ATP hydrolysis by MutS homologues is required for the function of these proteins in mismatch repair. However, the function of ATP hydrolysis in the repair reaction is not very clear. We have examined the role of ATP hydrolysis in oligomerization of Thermus aquaticus (Taq) MutS protein in solution. Analytical gel filtration and cross-linking of MutS protein with disuccinimidyl suburate suggest t...
متن کاملDNA mismatch repair.
DNA mismatch repair (MMR) is an evolutionarily conserved process that corrects mismatches generated during DNA replication and escape proofreading. MMR proteins also participate in many other DNA transactions, such that inactivation of MMR can have wide-ranging biological consequences, which can be either beneficial or detrimental. We begin this review by briefly considering the multiple functi...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1975
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.72.6.2202